Mab NFPRa
Catalog Fields
Clone ID/Product Name: Mab NFPRa
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: FPR1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2943552
Uniprot ID: P21462
Entrez Gene ID: 2357
Clonality: Monoclonal
Immunogen: rHis-FPR1
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: 3PU1
Epitope Mapped: Yes
Antigen Name: N-formyl peptide (fMLF) receptor, juxtamembrane C-terminus
Epitope Location or Sequence: C-terminal region 305-GQDFRERLI-313
Alternate Antigen Name:
Deposit Date: 8/7/2019
Antigen Molecular Weight: ~60 kDa
Depositor: Jesaitis, A.J.
Antigen Sequence:
Depositor Institution: Montana State University
Antigen Species: Human
Depositor Notes: The juxtamembrane epitope recognized by NFPRa is conserved in FPR1 and FPR2 (formerly FPRL1; 45 kDa) and is located on the cytoplasmic face of the receptor. Its recognition by NFPRa is insensitive to phosphorylation of either receptor.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: This antibody was first published as NFPR1 and was changed as FPR protein nomenclature evolved.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab NFPRa was deposited to the DSHB by Jesaitis, A.J. (DSHB Hybridoma Product Mab NFPRa)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
4 References
Initial Publication
C-terminal tail phosphorylation of N-formyl peptide receptor: differential recognition of two neutrophil chemoattractant receptors by monoclonal antibodies NFPR1 and NFPR2.
Jesaitis AJ
Journal of immunology (Baltimore, Md. : 1950) 179.4 (2007 Aug 15): 2520-31.