Mab H7
Catalog Fields
Clone ID/Product Name: Mab H7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: CAMP
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2943550
Uniprot ID: P49913
Entrez Gene ID: 820
Clonality: Monoclonal
Immunogen: Purified heterodimer Cytochrome b
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: 3PU1
Epitope Mapped: Yes
Antigen Name: cathelicidin anti-microbial protein (hCAP-18)
Epitope Location or Sequence: 115-N_ARG_Z-121 (Z=aromatic residue)
Alternate Antigen Name:
Deposit Date: 8/7/2019
Antigen Molecular Weight: 18 kDa
Depositor: Jesaitis, A.J.
Antigen Sequence:
Depositor Institution: Montana State University
Antigen Species: Human
Depositor Notes: This antigen peptide binds to bacterial lipopolysaccharides (LPS).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab H7 was deposited to the DSHB by Jesaitis, A.J. (DSHB Hybridoma Product Mab H7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
1 Reference
Initial Publication
Localization of hCAP-18 on the surface of chemoattractant-stimulated human granulocytes: analysis using two novel hCAP-18-specific monoclonal antibodies.
Jesaitis AJ
Journal of leukocyte biology 82.1 (2007 Jul): 161-72.