MW7
Catalog Fields
Clone ID/Product Name: MW7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: HTT
Ab Isotype: MIgM
Gene Name:
Entrez Gene ID: 3064
Clonality: Monoclonal
Immunogen: Recombinant Hd exon-1 67Q aggregate
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: HL-1
Epitope Mapped: Yes
Antigen Name: huntingtin
Epitope Location or Sequence: N-terminal, polyP
Alternate Antigen Name:
Deposit Date: 1/23/2003
Antigen Molecular Weight: Apparent: 348 kDa
Depositor: Patterson, P.H.
Antigen Sequence:
Depositor Institution: California Institute of Technology
Antigen Species: Human
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information: Western blot: in Human, weak binding to high molecular weight Htt, and strong binding to two smaller protein bands (doesn't differentiate between HD and non-HD); in mice, preferential strong binding to expanded polyQ domain. ICC: perinuclear or nuclear membranes in WT and expanded polyQ domain mutant; stains aggregates as inclusions in the nuclei of expanded polyQ domain mutant.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MW7 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product MW7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
10 References
Initial Publication
New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins.
Patterson PH
Brain research bulletin 56.3-4 (2001 Oct-Nov 1): 319-29.