MB1a(4A8)
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Catalog Fields
Clone ID/Product Name: MB1a(4A8)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: MBNL1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2618248
Uniprot ID: Q9NR56
Entrez Gene ID: 4154
Clonality: Monoclonal
Immunogen: His-tagged recombinant MBNL1 expressed in E. coli
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Antigen Name: Muscleblind-like splicing regulator 1
Epitope Location or Sequence: a.a. VSPSL/ linker between two zinc fingers in MBNL1 exon 3
Alternate Antigen Name:
Deposit Date: 10/11/2013
Antigen Molecular Weight: 42 kDa
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: Human
Depositor Notes: MB1a(4A8) does not cross-react with MBNL2 or MBNL3 isoforms.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information: RRID:AB_2618248
Additional Characterization: http://www.glennmorris.org.uk/datasheets/MB1a(4A8).doc
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MB1a(4A8) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MB1a(4A8))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Defective mRNA in myotonic dystrophy accumulates at the periphery of nuclear splicing speckles.
Morris GE
Genes to cells : devoted to molecular & cellular mechanisms 12.9 (2007 Sep): 1035-48.