M17/4.4.11.9
Catalog Fields
Clone ID/Product Name: M17/4.4.11.9
Available to For-Profits: Yes
Alternate Antibody Name: M17/4
Gene Symbol: Itgal
Ab Isotype: RIgG2a, kappa light chain
Gene Name:
Antibody Registry ID: AB_2280611
Uniprot ID: P24063
Entrez Gene ID: 16408
Clonality: Monoclonal
Immunogen: Anti-P815 cytotoxic T-lymphocytes from C57BL/6J mice.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS1 or P3X63Ag8.6.5.3
Epitope Mapped: Yes
Antigen Name: CD11a (Integrin alpha-L, LFA-1 alpha subunit), murine
Epitope Location or Sequence: I domain (aa 248-303)
Alternate Antigen Name:
Deposit Date: 4/4/1989
Antigen Molecular Weight: Predicted: 128 kDa; Apparent: 180 kDa
Depositor: Springer, T.A.
Antigen Sequence:
Depositor Institution: Center for Blood Research, Boston
Antigen Species: Mouse
Depositor Notes: Does not cross react with human antigen. Binds to all leukocytes except resident peritoneal macrophages. Doesn't appear to work for western blotting.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information:
Recommended Applications: FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M17/4.4.11.9 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product M17/4.4.11.9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship.
Springer TA
Cellular immunology 73.1 (1982 Oct): 1-11.