LC1
Catalog Fields
Clone ID/Product Name: LC1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: TPM3
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_10572389
Uniprot ID: P06753
Entrez Gene ID: 7170
Clonality: Monoclonal
Immunogen: Recombinant human tropomyosin isoform 5 and 4 fusion (hTM5/4) protein (N-terminal half of hTM5 and C-terminal half of hTM4).
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: Tropomyosin, alpha-3 chain (human)
Epitope Location or Sequence: First ten aa of the human TPM3 exon 1b product
Alternate Antigen Name:
Deposit Date: 7/17/2009
Antigen Molecular Weight: Apparent: 32 kDa
Depositor: Lin, J.J.-C.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Human
Depositor Notes: Recognizes Human non-muscle tropomyosin (hTM5), hamster (CHO) but not mouse.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Hamster, Human
Additional Information: Doesn't cross-react with rat or mouse tropomyosin.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LC1 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product LC1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
14 References
Initial Publication
Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
Lin JJ
The Journal of cell biology 129.3 (1995 May): 697-708.