K65/35
Catalog Fields
Clone ID/Product Name: K65/35
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Cntnap1
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2877274
Uniprot ID: P97846
Entrez Gene ID: 84008
Clonality: Monoclonal
Immunogen: Recombinant rat cytoplasmic domain GST fusion protein
Clone:
Immunogen Sequence: a.a.1308-1381 (cytoplasmic)
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Contactin-associated protein 1/ CASPR/Neurexin IV
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/21/2019
Antigen Molecular Weight: 220 kDa
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: Rat
Depositor Notes: Caspr KO sample courtesy of Drs Ido Horresh and Ori Peles, The Weizmann Institute of Science.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information:
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K65/35 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product K65/35)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
12 References
Initial Publication
Subunit composition and novel localization of K+ channels in spinal cord.
Trimmer JS
The Journal of comparative neurology 429.1 (2001 Jan 1): 166-76.