IIH6 C4
Catalog Fields
Clone ID/Product Name: IIH6 C4
Available to For-Profits: Yes
Alternate Antibody Name: IIH6
Gene Symbol: DAG1
Ab Isotype: MIgM, kappa light chain
Gene Name:
Antibody Registry ID: AB_2617216
Uniprot ID: Q28685
Entrez Gene ID: 100009278
Clonality: Monoclonal
Immunogen: Dystrophin complex isolated from detergent-solubilized skeletal muscle membranes using wheat-germ agglutinin-Sepharose.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Antigen Name: Dystroglycan, alpha
Epitope Location or Sequence: N-terminal region (lamiminin-binding glycoepitope)
Alternate Antigen Name:
Deposit Date: 9/29/2011
Antigen Molecular Weight: Apparent: 156 kDa
Depositor: Campbell, K.P.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Rabbit
Depositor Notes: This antibody binds to an extracellular peripheral glycoprotein that is associated with beta-dystroglycan, a transmembrane protein.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Canine, Human, Mouse, Rabbit, Rat
Additional Information: IIH6 C4 recognizes a glycoconjugate on the LARGE fully glycosylated form of alpha DAG. It detects the laminin-binding glycoepitope. Dystroglycan is a central component of the dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. RRID:AB_2617216
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IIH6 C4 was deposited to the DSHB by Campbell, K.P. (DSHB Hybridoma Product IIH6 C4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
35 References
Initial Publication
Membrane organization of the dystrophin-glycoprotein complex.
Campbell KP
Cell 66.6 (1991 Sep 20): 1121-31.