DCSP-2 (6D6)

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SKU: DCSP-2 (6D6)

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Supernatant 1.0 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Clone ID/Product Name: DCSP-2 (6D6)

Available to For-Profits: Yes

Alternate Antibody Name:

Gene Symbol: Csp

Ab Isotype: MIgG2b

Gene Name:

Antibody Registry ID: AB_528183

Uniprot ID: Q03751

RRID:

Entrez Gene ID: 40459

Clonality: Monoclonal

Immunogen: Recombinant CSP/GST fusion protein

Clone:

Immunogen Sequence: Full length protein

Myeloma Strain:

Epitope Mapped: Yes

Antigen Name: Cysteine string protein

Epitope Location or Sequence: REQAGGQP (1.1. 218-222)

Alternate Antigen Name:

Deposit Date: 4/27/2005

Antigen Molecular Weight: Multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-2: 32, 33, 34, and 36 kDa.

Depositor: Benzer, S.

Antigen Sequence:

Depositor Institution: California Institute of Technology, Division of Biology

Antigen Species: Drosophila

Depositor Notes:

Host Species: mouse

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: Drosophila

Additional Information: This antibody recognizes all known CSP isoforms and labels all synapses. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. DCSP-2 is often used as a synaptic vesicle marker. CSP is also known as DnaJ homolog subfamily C member 5 homolog. RRID: AB_528183 . FBgn0004179

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

DCSP-2 (6D6) was deposited to the DSHB by Benzer, S. (DSHB Hybridoma Product DCSP-2 (6D6))

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

34 References

Initial Publication

IF References

WB References

IHC References

Epitope Map References

All References

Initial Publication

Paralysis and early death in cysteine string protein mutants of Drosophila.
Benzer S
Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

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DCSP-2 (6D6)

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