DA5D6
Catalog Fields
Clone ID/Product Name: DA5D6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: pdgfra
Ab Isotype: MIgG1
Gene Name:
Uniprot ID: P26619
Entrez Gene ID: 380245
Clonality: Monoclonal
Immunogen: Bacterial fusion protein
Clone:
Immunogen Sequence: Cytoplasmic domain produced in BL21
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Platelet-derived growth factor receptor alpha
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/3/2021
Antigen Molecular Weight: 122.7 kDa
Depositor: Alfandari, D.
Antigen Sequence:
Depositor Institution: University of Massachusetts, Amherst
Antigen Species: Xenopus
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Xenopus
Additional Information: Please reference NIH Grant R24OD21485 in alll publications.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DA5D6 was deposited to the DSHB by Alfandari, D. (DSHB Hybridoma Product DA5D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.