D3
Catalog Fields
Clone ID/Product Name: D3
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: DES
Ab Isotype: MIgA, kappa light chain
Gene Name:
Entrez Gene ID: 395906
Clonality: Monoclonal
Immunogen: Acetic acid solubilized demins from chicken gizzard
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2
Epitope Mapped: Yes
Antigen Name: Desmin, intermediate filament
Epitope Location or Sequence: N-terminal rod domain
Alternate Antigen Name:
Deposit Date: 4/22/1986
Antigen Molecular Weight: 53.2 kDa
Depositor: Fischman, D.A.
Antigen Sequence:
Depositor Institution: Cornell University Medical College
Antigen Species: Chicken
Depositor Notes: The D3 antibody is positive in stage 26 chick limb bud, day 19 rat limb bud and day 12 rat myotome. D3 also stained primary cultures of embryonic cardiac myocytes and H.H. stage 17-18 embryonic chick hearts.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Hamster, Mouse, Rat
Additional Information: RID:AB_528195
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D3 was deposited to the DSHB by Fischman, D.A. (DSHB Hybridoma Product D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Immunocytochemical analysis of intermediate filaments in embryonic heart cells with monoclonal antibodies to desmin.
Fischman DA
The Journal of cell biology 98.6 (1984 Jun): 2179-91.