CWK-F12
Catalog Fields
Clone ID/Product Name: CWK-F12
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: ESR2
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_2722105
Uniprot ID: Q92731
Entrez Gene ID: 2100
Clonality: Monoclonal
Immunogen: E2 ligand-binding domain
Clone:
Immunogen Sequence: a.a. 256-505
Myeloma Strain: SP 2/0
Epitope Mapped: No
Antigen Name: Estrogen receptor beta (ER 2)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/30/2017
Antigen Molecular Weight: 59 kDa
Depositor: Katzenellenbogen, B.S.
Antigen Sequence:
Depositor Institution: University of Illinois
Antigen Species: Human
Depositor Notes: This monoclonal antibody has recently be shown to be specific for ER beta unlike other commonly used anti-ER beta antibodies (see Nelson et al 2017 PMID 27889472).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Mouse, Porcine, Rat
Additional Information: RRID: AB_2722105. This antibody has also been validated for rapid immunoprecipitation mass spectrometry (RIME).
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CWK-F12 was deposited to the DSHB by Katzenellenbogen, B.S. (DSHB Hybridoma Product CWK-F12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
19 References
Initial Publication
Human estrogen receptor beta-specific monoclonal antibodies: characterization and use in studies of estrogen receptor beta protein expression in reproductive tissues.
Katzenellenbogen BS
Molecular and cellular endocrinology 181.1-2 (2001 Jul 5): 139-50.
On the problem of oncogene of tumour viruses.
Mekler LB
Acta virologica 19.6 (1975 Nov): 501-8.