CT-4

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SKU: CT-4-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: CT-4
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: CD4
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2796427 
Uniprot ID: Q6Q4G2 
RRID:  
Entrez Gene ID: 395362 
Clonality: Monoclonal
Immunogen: Chicken thymocytes and Ig-negative blood leukocytes
Clone:
Immunogen Sequence:
Myeloma Strain: P3X63.Ag8.653
Epitope Mapped: No
Antigen Name: CD4, chick
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/21/2023
Antigen Molecular Weight: 52.2 kDa
Depositor: UAB Research Foundation
Antigen Sequence:
Depositor Institution: U of Alabama, Birmingham
Antigen Species: Chick
Depositor Notes: Hybridoma developed by M.D. Cooper.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chick, Pigeon, Quail, Turkey
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CT-4 was deposited to the DSHB by UAB Research Foundation (DSHB Hybridoma Product CT-4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

3 References

  • Initial Publication
  • IHC References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication

    Identification of the avian homologues of mammalian CD4 and CD8 antigens.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 140.7 (1988 Apr 1): 2133-8.

    IHC References
    IP References

    Identification of the avian homologues of mammalian CD4 and CD8 antigens.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 140.7 (1988 Apr 1): 2133-8.

    FB References

    Identification of the avian homologues of mammalian CD4 and CD8 antigens.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 140.7 (1988 Apr 1): 2133-8.

    FACS References

    Identification of the avian homologues of mammalian CD4 and CD8 antigens.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 140.7 (1988 Apr 1): 2133-8.

    All References

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