CK-5D11
Catalog Fields
Clone ID/Product Name: CK-5D11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: CKM
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_1553661
Uniprot ID: P06732
Entrez Gene ID: 1158
Clonality: Monoclonal
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: creatine kinase
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/25/2008
Antigen Molecular Weight: 43 kDa
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: human
Depositor Notes: This antibody does not Western Blot, itæcaptures native MM-CK from muscle extract. It does not work with rabbits, rats, pigs, or chicken.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. RRID:AB_1553661
Additional Characterization: http://www.glennmorris.org.uk/datasheets/CK-5D11.doc
Recommended Applications: Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CK-5D11 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product CK-5D11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Treatment of human muscle creatine kinase with glutaraldehyde preferentially increases the immunogenicity of the native conformation and permits production of high-affinity monoclonal antibodies which recognize two distinct surface epitopes.
Morris GE
Journal of immunological methods 125.1-2 (1989 Dec 20): 251-9.