Anti-vasa [Rb-IgG]
![Anti-vasa [Rb-IgG]](https://dshb.biology.uiowa.edu/scs/img/2023_Logomark_Circle-Histo.png?resizeid=3&resizeh=600&resizew=600)
Catalog Fields
Clone ID/Product Name: Anti-vasa [Rb-IgG]
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: Vas
Ab Isotype: Rabbit IgG
Gene Name:
Entrez Gene ID: 26067080
Clonality: Polyclonal
Immunogen: amino acids 1-260 D. melanogaster vasa
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Vasa
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/17/2024
Antigen Molecular Weight: 32.88 kDa
Depositor: Shu Yuan, Yang
Antigen Sequence:
Depositor Institution: Chang Gung University
Antigen Species: Drosophila
Depositor Notes:
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Anti-vasa [Rb-IgG] was deposited to the DSHB by Shu Yuan, Yang (DSHB Hybridoma Product Anti-vasa [Rb-IgG])
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
1 Reference
Initial Publication
Transient chromatin decompaction at the start of D. melanogaster male embryonic germline development.
Yang SY
Life science alliance 7.10 (2024 Oct): .