Anti-RBFOX3 [BCN21.2.2B9]
![Anti-RBFOX3 [BCN21.2.2B9]](https://dshb.biology.uiowa.edu/scs/img/2023_Logomark_Circle-Histo.png?resizeid=3&resizeh=600&resizew=600)
Catalog Fields
Clone ID/Product Name: Anti-RBFOX3 [BCN21.2.2B9]
Available to For-Profits: No
Alternate Antibody Name: BCN21.2.2B9
Gene Symbol: RBFOX3
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_3083095
Uniprot ID: A6NFN3
Entrez Gene ID: 146713
Clonality: Monoclonal
Immunogen: Recombinant protein produced in yeast
Clone:
Immunogen Sequence: Recombinant fragment of human RBFOX3 protein (aa 28-158)
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: RNA binding protein fox-1 homolog 3/ Neun
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/31/2023
Antigen Molecular Weight: 33.8 kDa
Depositor: Pino, I.
Antigen Sequence:
Depositor Institution: CDI Labs
Antigen Species: Human
Depositor Notes: Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information: Mouse strain CD1 was used.
Predicted Species Reactivity:
Human Protein Atlas: https://www.proteinatlas.org/ENSG00000167281-RBFOX3
Recommended Applications: Immunofluorescence, Microarray
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Anti-RBFOX3 [BCN21.2.2B9] was deposited to the DSHB by Pino, I. (DSHB Hybridoma Product Anti-RBFOX3 [BCN21.2.2B9])
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
1 Reference
Initial Publication
Basic principles of hydrogel-based tissue transformation technologies and their applications.
Chung K
Cell 184.16 (2021 Aug 5): 4115-4136.