Anti-CALB2 [BCN4.2.3B2]

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SKU: Anti-CALB2 [BCN4.2.3B2]

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: Anti-CALB2 [BCN4.2.3B2]
Available to For-Profits: No
Alternate Antibody Name: BCN4.2.3B2
Gene Symbol: CALB2
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_3073529 
Uniprot ID: P22676 
RRID:  
Entrez Gene ID: 794 
Clonality: Monoclonal
Immunogen: Recombinant protein produced in yeast
Clone:
Immunogen Sequence: Full length human sequence
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: Calretinin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/15/2022
Antigen Molecular Weight: 31.5 kDa
Depositor: Pino, I.
Antigen Sequence:
Depositor Institution: CDI Labs
Antigen Species: Human
Depositor Notes: Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information: Mouse strain CD1 was used.
Predicted Species Reactivity:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Microarray
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Anti-CALB2 [BCN4.2.3B2] was deposited to the DSHB by Pino, I. (DSHB Hybridoma Product Anti-CALB2 [BCN4.2.3B2])
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.

1 Reference

  • Initial Publication
  • Epitope Map References
  • All References
  • Initial Publication
    Epitope Map References
    All References

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