7D8 PI-9
Catalog Fields
Clone ID/Product Name: 7D8 PI-9
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: SERPINB9
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2936809
Uniprot ID: P50453
Entrez Gene ID: 5272
Clonality: Monoclonal
Immunogen: Recombinant protein
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: SERPINB9 / PI-9
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/26/2023
Antigen Molecular Weight: 42.4 kDa
Depositor: Bird, P.I.
Antigen Sequence:
Depositor Institution: Monash University, Melbourne, AU
Antigen Species: Human
Depositor Notes: Serpinb9 is an intracellular inhibitor of the cytoxic protease granzyme B found in (but not limited to) CD8+ T cells, natural killer cells, mast cells and CD8+ dendritic cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: Recognizes a unique epitope not present on related family B / ovalbumin serpins.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7D8 PI-9 was deposited to the DSHB by Bird, P.I. (DSHB Hybridoma Product 7D8 PI-9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
2 References
Initial Publication
Perforin-independent expression of granzyme B and proteinase inhibitor 9 in human testis and placenta suggests a role for granzyme B-mediated proteolysis in reproduction.
Bird PI
Molecular human reproduction 7.12 (2001 Dec): 1133-42.