7A6
Catalog Fields
Clone ID/Product Name: 7A6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: NFATC1
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2152505
Uniprot ID: O95644
Entrez Gene ID: 4772
Clonality: Monoclonal
Immunogen: Recombinant GST/NFATC1 (aa 1-654 of isoform A-alpha) fusion protein
Clone:
Immunogen Sequence: partial protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Nuclear factor of activated T-cells, cytoplasmic 1
Epitope Location or Sequence: aa 186-380
Alternate Antigen Name:
Deposit Date: 9/17/2003
Antigen Molecular Weight: Predicted: multiple isoforms from 39 to 101 kDa; Apparent: phosphorylated form (cytoplasmic) at 150, 120, and 80 kDa, and dephosphorylated form (nuclear) at 120, 80, and 68 kDa
Depositor: Crabtree, G.R.
Antigen Sequence:
Depositor Institution: Stanford University
Antigen Species: human
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information:
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7A6 was deposited to the DSHB by Crabtree, G.R. (DSHB Hybridoma Product 7A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
NF-AT components define a family of transcription factors targeted in T-cell activation.
Crabtree GR
Nature 369.6480 (1994 Jun 9): 497-502.