4A1
Catalog Fields
Clone ID/Product Name: 4A1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2732839
Uniprot ID: W4XBP7
Clonality: Monoclonal
Immunogen: 15S complex dynein subunits from axonemes
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: P3UI
Epitope Mapped: No
Antigen Name: Tubulin, alpha
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/7/2018
Antigen Molecular Weight: 51 kDa
Depositor: Fuller, M.T.
Antigen Sequence:
Depositor Institution: Stanford University
Antigen Species: Sea Urchin S. purpuratus
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans, Drosophila, Mouse, S. pombe, Saccharomyces cerevisiae, Trypanosomes
Additional Information: RRID:AB_2732839
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4A1 was deposited to the DSHB by Fuller, M.T. (DSHB Hybridoma Product 4A1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
18 References
Initial Publication
A cytoplasmic dynein motor in Drosophila: identification and localization during embryogenesis.
McIntosh JR
Journal of cell science 107 ( Pt 6). (1994 Jun): 1557-69.