34B3 anti-Fasciclin II
Catalog Fields
Clone ID/Product Name: 34B3 anti-Fasciclin II
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Fas2
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 31364
Clonality: Monoclonal
Immunogen: recombinant fusion protein, trpE fused to two different, nonoverlapping fragments of Drosophila fasII cDNA: a 1227 bp XhoI-HindIII fragment encopassing Ig domains 2-5 and a 496 bp SalI-ClaI fragment including fibronectin domain 2.
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3X63.Ag8.653
Epitope Mapped: Yes
Antigen Name: fasciclin II (Drosophila, all isoforms)
Epitope Location or Sequence: extracellular domain of all four isoforms
Alternate Antigen Name:
Deposit Date: 11/1/2001
Antigen Molecular Weight: Predicted: three main isoforms of 97, 90, and 86 kDa
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: Extracellular cell binding.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: The initial publication and the immunogen information are only putative and may or may not be correct. The depositor didn't specify any initial publication and didn't indicate the immunogen used. The mAb described in [PMID 1913818] has the generic name: anti-fasciclin II Mab.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
34B3 anti-Fasciclin II was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 34B3 anti-Fasciclin II)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
7 References
Initial Publication
Genetic analysis of growth cone guidance in Drosophila: fasciclin II functions as a neuronal recognition molecule.
Goodman CS
Cell 67.1 (1991 Oct 4): 45-57.