22/18
Catalog Fields
Product Name/ID: 22/18
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_531815
Clonality: Monoclonal
Immunogen: early to mid-bud stage blastemas
Clone:
Immunogen Sequence:
Myeloma Strain: NS1/SP2
Epitope Mapped: No
Antigen Name: blastemal regeneration cell marker of the newt
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/1/1987
Antigen Molecular Weight:
Depositor: Brockes, J.P.
Antigen Sequence:
Depositor Institution: Ludwig Institute for Cancer Research, Middlesex Hospital, University College Branch
Antigen Species: newt
Depositor Notes: Western blot was unsuccessful. For immunohistology see papers of developing and regenerating limbs and normal embryos. The antibody recognizes a cytoplasmic component of a population of blastemal cells that is lost when the cells differentiate into mature tissues. The growth of this population of cells is nerve dependent. (Intermediate filament protein or intermediate filament associated protein when staining fixed cultured blastemal cells.)
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Axolotl, Newt
Additional Information: The immunization used 50-100 micrograms of protein for priming and for several boost for sacrificing. The origin of the antibody is notophthalmus viridescens. Works with Notopthalmus and Pleurodeles. Axolotl is weak. Triturus is weak. Nothing in higher vertebrates. RRID:AB_531815
Recommended Applications: Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
22/18 was deposited to the DSHB by Brockes, J.P. (DSHB Hybridoma Product 22/18)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
5 References
Initial Publication
Monoclonal antibodies identify blastemal cells derived from dedifferentiating limb regeneration.
Brockes JP
Nature 308.5954 (1984 Mar 1-7): 67-9.