11-26c
Catalog Fields
Clone ID/Product Name: 11-26c
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: Ighd
Ab Isotype: RIgG2a, kappa light chain
Gene Name:
Antibody Registry ID: AB_2794607
Uniprot ID: P01881
Entrez Gene ID: 380797
Clonality: Monoclonal
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain: P3X63.Ag8.653
Epitope Mapped: No
Antigen Name: Mouse IgD
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/21/2023
Antigen Molecular Weight: 28.3 kDa
Depositor: UAB Research Foundation
Antigen Sequence:
Depositor Institution: U of Alabama, Birmingham
Antigen Species: Mouse
Depositor Notes: Hybridoma developed by J.F.Kearney.
Host Species: rat
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse
Additional Information:
Recommended Applications: FFPE, Function Blocking, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
11-26c was deposited to the DSHB by UAB Research Foundation (DSHB Hybridoma Product 11-26c)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
3 References
Initial Publication
Normal induction but attenuated progression of germinal center responses in BAFF and BAFF-R signaling-deficient mice.
Manser T
The Journal of experimental medicine 198.8 (2003 Oct 20): 1157-69.