SV2
Catalog Fields
Product Name/ID: SV2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: SV2A
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2315387
Uniprot ID: Q7L0J3
Entrez Gene ID: 9900
Clonality: Monoclonal
Immunogen: Purified synaptic vesicles
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Synaptic vesicle glycoprotein 2A; synaptic vesicles
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/19/1996
Antigen Molecular Weight: 95 kDa
Depositor: Buckley, K.M.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Ommata
Depositor Notes: This antibody recognizes all three isoforms: SV2A, SV2B and SV2C.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian, Bovine, Fish, Human, Mouse, Planaria, Primate, Rat, Xenopus, Zebrafish
Additional Information: recognizes all 3 isoforms, SV2A, B & C
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SV2 was deposited to the DSHB by Buckley, K.M. (DSHB Hybridoma Product SV2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
43 References
Initial Publication
Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells.
Kelly RB
The Journal of cell biology 100.4 (1985 Apr): 1284-94.