G3G4 (anti-BrdU)
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Catalog Fields
Product Name/ID: G3G4 (anti-BrdU)
Available to For-Profits: Yes
Alternate Antibody Name: G3G4(AntiBrdUrd), Bromodeoxyuridine
Gene Name: BrdU
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2314035
Clonality: Monoclonal
Immunogen: 5-bromo-2'-deoxyuridine-BSA
Clone:
Immunogen Sequence:
Myeloma Strain: Sp 2/0 Ag 14
Epitope Mapped: No
Antigen Name: 5-bromo-2'-deoxyuridine BrdU
Epitope Location or Sequence:
Alternate Antigen Name: Proliferation Marker
Deposit Date: 10/5/1993
Antigen Molecular Weight:
Depositor: Kaufman, S.J.
Antigen Sequence:
Depositor Institution: University of Illinois
Antigen Species: Not applicable
Depositor Notes: This antibody detects BrdU incorporated into cell nuclei during S-phase. 2N HCL treatment of nuclei increases antibody access to incorporated BrdU within the DNA (see Foster et al., 1987 for details).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: All species labeled with BrdU
Additional Information: IHC paper: Acta zoologica doi: 10.1111/azo.12114
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
G3G4 (anti-BrdU) was deposited to the DSHB by Kaufman, S.J. (DSHB Hybridoma Product G3G4 (anti-BrdU))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
43 References
Initial Publication
A laminin substrate promotes myogenesis in rat skeletal muscle cultures: analysis of replication and development using antidesmin and anti-BrdUrd monoclonal antibodies.
Kaufman SJ
Developmental biology 122.1 (1987 Jul): 11-20.