R4B
Catalog Fields
Product Name/ID: R4B
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Reln
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_1157892
Uniprot ID: Q60841
Entrez Gene ID: 19699
Clonality: Monoclonal
Immunogen: Reelin protein domain a.a. 1796-2115
Clone:
Immunogen Sequence: Synthetic peptide-BSA conjugate
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Reelin
Epitope Location or Sequence: Central region a.a. 1810-1825
Alternate Antigen Name:
Deposit Date: 7/2/2008
Antigen Molecular Weight: 387.5 kDa
Depositor: Goffinet, A.
Antigen Sequence:
Depositor Institution: University of Louvain, Belgium
Antigen Species: Mouse
Depositor Notes: R4B was generated in Reln-Orl mutant mice.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rodent
Additional Information: RRID:AB_1157892
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
R4B was deposited to the DSHB by Goffinet, A. (DSHB Hybridoma Product R4B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
9 References
Initial Publication
Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
Goffinet AM
The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.