8.1.1

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Product Name/ID: 8.1.1
Available to For-Profits: Yes
Alternate Antibody Name: glycoprotein, 38 kDa, mucin-like
Gene Name: Pdpn
Ab Isotype: Syrian Hamster IgG
Gene Symbol:
Antibody Registry ID: AB_531893 
Uniprot ID: Q62011 
RRID:  
Entrez Gene ID: 14726 
Clonality: Monoclonal
Immunogen: Thymic stromal cell line E-71 (murine).
Clone:
Immunogen Sequence:
Myeloma Strain: P3x
Epitope Mapped: No
Antigen Name: Podoplanin
Epitope Location or Sequence: extracellular domain
Alternate Antigen Name:
Deposit Date: 6/7/2001
Antigen Molecular Weight: 38 kDa (36-40 kDa)
Depositor: Farr, A.G .
Antigen Sequence:
Depositor Institution: University of Washington
Antigen Species: Mouse
Depositor Notes: Reacts with T1 alpha in the lung, podoplanin in the kidney, and lymphatic endothelium; Recognizes aggrus/podoplanin expressed by platelets; Stains formalin-fixed paraffin embedded samples.
Host Species: Syrian hamster
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8.1.1 was deposited to the DSHB by Farr, A.G . (DSHB Hybridoma Product 8.1.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

48 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • FACS References
  • All References
    • Initial Publication

    Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues.
    Aruffo A
    The Journal of experimental medicine 176.5 (1992 Nov 1): 1477-82.

    IF References

    Mammalian Nkx2.2+ perineurial glia are essential for motor nerve development.
    Kucenas S
    Developmental dynamics : an official publication of the American Association of Anatomists 243.9 (2014 Sep): 1116-29.

    Chronic high-fat diet impairs collecting lymphatic vessel function in mice.
    Detmar M
    PloS one 9.4 (2014): e94713.

    An important role of the SDF-1/CXCR4 axis in chronic skin inflammation.
    Detmar M
    PloS one 9.4 (2014): e93665.

    Epithelial β1 integrin is required for lung branching morphogenesis and alveolarization.
    Zent R
    Development (Cambridge, England) 141.24 (2014 Dec): 4751-62.

    Two nested developmental waves demarcate a compartment boundary in the mouse lung.
    Chen J
    Nature communications 5. (2014 May 29): 3923.

    Thymus cell antigen 1 (Thy1, CD90) is expressed by lymphatic vessels and mediates cell adhesion to lymphatic endothelium.
    Detmar M
    Experimental cell research 316.17 (2010 Oct 15): 2982-92.

    Notch-dependent differentiation of adult airway basal stem cells.
    Hogan BL
    Cell stem cell 8.6 (2011 Jun 3): 639-48.

    Dlg5 maintains apical aPKC and regulates progenitor differentiation during lung morphogenesis.
    Vasioukhin V
    Developmental biology 377.2 (2013 May 15): 375-84.

    Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2.
    Xia L
    Nature 502.7469 (2013 Oct 3): 105-9.

    Alveolar epithelial cell therapy with human cord blood-derived hematopoietic progenitor cells.
    Padbury JF
    The American journal of pathology 178.3 (2011 Mar): 1329-39.

    Expansion of cortical and medullary sinuses restrains lymph node hypertrophy during prolonged inflammation.
    Angeli V
    Journal of immunology (Baltimore, Md. : 1950) 188.8 (2012 Apr 15): 4065-80.

    Sox2 activates cell proliferation and differentiation in the respiratory epithelium.
    Whitsett JA
    American journal of respiratory cell and molecular biology 45.1 (2011 Jul): 101-10.

    The chemokine CX3CL1 promotes trafficking of dendritic cells through inflamed lymphatics.
    Jackson DG
    Journal of cell science 126.Pt 22 (2013 Nov 15): 5259-70.

    Blocking Fibroblast Growth Factor receptor signaling inhibits tumor growth, lymphangiogenesis, and metastasis.
    Auguste P
    PloS one 7.6 (2012): e39540.

    Netrin-4 induces lymphangiogenesis in vivo.
    Li DY
    Blood 115.26 (2010 Jul 1): 5418-26.

    CD200-CD200R signaling suppresses anti-tumor responses independently of CD200 expression on the tumor.
    Meyaard L
    Oncogene 31.24 (2012 Jun 14): 2979-88.

    Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo.
    Schüler T
    European journal of immunology 40.9 (2010 Sep): 2391-400.

    β-Catenin-SOX2 signaling regulates the fate of developing airway epithelium.
    Stripp BR
    Journal of cell science 125.Pt 4 (2012 Feb 15): 932-42.

    An Adaptor Molecule Afadin Regulates Lymphangiogenesis by Modulating RhoA Activity in the Developing Mouse Embryo.
    Ogita H
    PloS one 8.6 (2013): e68134.

    Lung mesenchymal expression of Sox9 plays a critical role in tracheal development.
    Warburton D
    BMC biology 11. (2013 Nov 25): 117.

    Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans.
    Luther SA
    The American journal of pathology 178.4 (2011 Apr): 1662-75.

    Reproducible isolation of lymph node stromal cells reveals site-dependent differences in fibroblastic reticular cells.
    Turley SJ
    Frontiers in immunology 2. (2011): 35.

    Remodeling of the lymphatic vasculature during mouse mammary gland morphogenesis is mediated via epithelial-derived lymphangiogenic stimuli.
    Harvey NL
    The American journal of pathology 181.6 (2012 Dec): 2225-38.

    Hyaluronan and TLR4 promote surfactant-protein-C-positive alveolar progenitor cell renewal and prevent severe pulmonary fibrosis in mice.
    Noble PW
    Nature medicine 22.11 (2016 Nov): 1285-1293.

    Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program.
    Mäkinen T
    Nature communications 9.1 (2018 Apr 17): 1511.

    Genetic link between renal birth defects and congenital heart disease.
    Pazour GJ
    Nature communications 7. (2016 Mar 22): 11103.

    A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting.
    Zhou B
    Cell stem cell 28.6 (2021 Jun 3): 1160-1176.e7.

    WB References

    Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus.
    Hosier S
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 40.5 (1992 May): 651-64.

    Wnt3a mitigates acute lung injury by reducing P2X7 receptor-mediated alveolar epithelial type I cell death.
    Liu L
    Cell death & disease 5.6 (2014 Jun 12): e1286.

    Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5.
    Bonewald LF
    Methods in molecular biology (Clifton, N.J.) 816. (2012): 67-81.

    Dlg5 maintains apical aPKC and regulates progenitor differentiation during lung morphogenesis.
    Vasioukhin V
    Developmental biology 377.2 (2013 May 15): 375-84.

    Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2.
    Xia L
    Nature 502.7469 (2013 Oct 3): 105-9.

    Serines in the intracellular tail of podoplanin (PDPN) regulate cell motility.
    Goldberg GS
    The Journal of biological chemistry 288.17 (2013 Apr 26): 12215-21.

    Plant lectin can target receptors containing sialic acid, exemplified by podoplanin, to inhibit transformed cell growth and migration.
    Goldberg GS
    PloS one 7.7 (2012): e41845.

    IHC References

    Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus.
    Hosier S
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 40.5 (1992 May): 651-64.

    Mammalian Nkx2.2+ perineurial glia are essential for motor nerve development.
    Kucenas S
    Developmental dynamics : an official publication of the American Association of Anatomists 243.9 (2014 Sep): 1116-29.

    Chronic high-fat diet impairs collecting lymphatic vessel function in mice.
    Detmar M
    PloS one 9.4 (2014): e94713.

    Thymus cell antigen 1 (Thy1, CD90) is expressed by lymphatic vessels and mediates cell adhesion to lymphatic endothelium.
    Detmar M
    Experimental cell research 316.17 (2010 Oct 15): 2982-92.

    Notch-dependent differentiation of adult airway basal stem cells.
    Hogan BL
    Cell stem cell 8.6 (2011 Jun 3): 639-48.

    Expansion of cortical and medullary sinuses restrains lymph node hypertrophy during prolonged inflammation.
    Angeli V
    Journal of immunology (Baltimore, Md. : 1950) 188.8 (2012 Apr 15): 4065-80.

    Blocking Fibroblast Growth Factor receptor signaling inhibits tumor growth, lymphangiogenesis, and metastasis.
    Auguste P
    PloS one 7.6 (2012): e39540.

    Plant lectin can target receptors containing sialic acid, exemplified by podoplanin, to inhibit transformed cell growth and migration.
    Goldberg GS
    PloS one 7.7 (2012): e41845.

    CCR7-expressing B16 melanoma cells downregulate interferon-γ-mediated inflammation and increase lymphangiogenesis in the tumor microenvironment.
    Hwang ST
    Oncogenesis 1.5 (2012 May 7): e9.

    Glucocorticoid activity during lung maturation is essential in mesenchymal and less in alveolar epithelial cells.
    Schütz G
    Molecular endocrinology (Baltimore, Md.) 25.8 (2011 Aug): 1280-8.

    ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells.
    Apte SS
    The American journal of pathology 176.3 (2010 Mar): 1494-504.

    Immunohistochemical detection and regulation of α5 nicotinic acetylcholine receptor (nAChR) subunits by FoxA2 during mouse lung organogenesis.
    Reynolds PR
    Respiratory research 12.1 (2011 Jun 17): 82.

    β-Catenin-SOX2 signaling regulates the fate of developing airway epithelium.
    Stripp BR
    Journal of cell science 125.Pt 4 (2012 Feb 15): 932-42.

    CD11c(hi) dendritic cells regulate the re-establishment of vascular quiescence and stabilization after immune stimulation of lymph nodes.
    Lu TT
    Journal of immunology (Baltimore, Md. : 1950) 184.8 (2010 Apr 15): 4247-57.

    Lung mesenchymal expression of Sox9 plays a critical role in tracheal development.
    Warburton D
    BMC biology 11. (2013 Nov 25): 117.

    Multiple roles and interactions of Tbx4 and Tbx5 in development of the respiratory system.
    Papaioannou VE
    PLoS genetics 8.8 (2012): e1002866.

    Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis.
    Schiller HB
    Nature communications 11.1 (2020 Jul 16): 3559.

    FFPE References

    Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis.
    Schiller HB
    Nature communications 11.1 (2020 Jul 16): 3559.

    FACS References

    Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus.
    Hosier S
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 40.5 (1992 May): 651-64.

    Thymus cell antigen 1 (Thy1, CD90) is expressed by lymphatic vessels and mediates cell adhesion to lymphatic endothelium.
    Detmar M
    Experimental cell research 316.17 (2010 Oct 15): 2982-92.

    Tissue inflammation modulates gene expression of lymphatic endothelial cells and dendritic cell migration in a stimulus-dependent manner.
    Halin C
    Blood 118.1 (2011 Jul 7): 205-15.

    Lymph node-resident lymphatic endothelial cells mediate peripheral tolerance via Aire-independent direct antigen presentation.
    Engelhard VH
    The Journal of experimental medicine 207.4 (2010 Apr 12): 681-8.

    Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions.
    Turley SJ
    The Journal of experimental medicine 207.4 (2010 Apr 12): 689-97.

    Expansion of cortical and medullary sinuses restrains lymph node hypertrophy during prolonged inflammation.
    Angeli V
    Journal of immunology (Baltimore, Md. : 1950) 188.8 (2012 Apr 15): 4065-80.

    Identification of salmonella pathogenicity island-2 type III secretion system effectors involved in intramacrophage replication of S. enterica serovar typhimurium: implications for rational vaccine design.
    Helaine S
    mBio 4.2 (2013 Apr 16): e00065.

    miR-31 functions as a negative regulator of lymphatic vascular lineage-specific differentiation in vitro and vascular development in vivo.
    Detmar M
    Molecular and cellular biology 30.14 (2010 Jul): 3620-34.

    Impaired thymic tolerance to α-myosin directs autoimmunity to the heart in mice and humans.
    Lipes MA
    The Journal of clinical investigation 121.4 (2011 Apr): 1561-73.

    Interleukin-7 is produced by afferent lymphatic vessels and supports lymphatic drainage.
    Halin C
    Blood 122.13 (2013 Sep 26): 2271-81.

    Novel role for ALCAM in lymphatic network formation and function.
    Halin C
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27.3 (2013 Mar): 978-90.

    Reproducible isolation of lymph node stromal cells reveals site-dependent differences in fibroblastic reticular cells.
    Turley SJ
    Frontiers in immunology 2. (2011): 35.

    All References

    Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus.
    Hosier S
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 40.5 (1992 May): 651-64.

    Mammalian Nkx2.2+ perineurial glia are essential for motor nerve development.
    Kucenas S
    Developmental dynamics : an official publication of the American Association of Anatomists 243.9 (2014 Sep): 1116-29.

    Chronic high-fat diet impairs collecting lymphatic vessel function in mice.
    Detmar M
    PloS one 9.4 (2014): e94713.

    Thymus cell antigen 1 (Thy1, CD90) is expressed by lymphatic vessels and mediates cell adhesion to lymphatic endothelium.
    Detmar M
    Experimental cell research 316.17 (2010 Oct 15): 2982-92.

    Notch-dependent differentiation of adult airway basal stem cells.
    Hogan BL
    Cell stem cell 8.6 (2011 Jun 3): 639-48.

    Expansion of cortical and medullary sinuses restrains lymph node hypertrophy during prolonged inflammation.
    Angeli V
    Journal of immunology (Baltimore, Md. : 1950) 188.8 (2012 Apr 15): 4065-80.

    Blocking Fibroblast Growth Factor receptor signaling inhibits tumor growth, lymphangiogenesis, and metastasis.
    Auguste P
    PloS one 7.6 (2012): e39540.

    Plant lectin can target receptors containing sialic acid, exemplified by podoplanin, to inhibit transformed cell growth and migration.
    Goldberg GS
    PloS one 7.7 (2012): e41845.

    CCR7-expressing B16 melanoma cells downregulate interferon-γ-mediated inflammation and increase lymphangiogenesis in the tumor microenvironment.
    Hwang ST
    Oncogenesis 1.5 (2012 May 7): e9.

    Glucocorticoid activity during lung maturation is essential in mesenchymal and less in alveolar epithelial cells.
    Schütz G
    Molecular endocrinology (Baltimore, Md.) 25.8 (2011 Aug): 1280-8.

    ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells.
    Apte SS
    The American journal of pathology 176.3 (2010 Mar): 1494-504.

    Immunohistochemical detection and regulation of α5 nicotinic acetylcholine receptor (nAChR) subunits by FoxA2 during mouse lung organogenesis.
    Reynolds PR
    Respiratory research 12.1 (2011 Jun 17): 82.

    β-Catenin-SOX2 signaling regulates the fate of developing airway epithelium.
    Stripp BR
    Journal of cell science 125.Pt 4 (2012 Feb 15): 932-42.

    CD11c(hi) dendritic cells regulate the re-establishment of vascular quiescence and stabilization after immune stimulation of lymph nodes.
    Lu TT
    Journal of immunology (Baltimore, Md. : 1950) 184.8 (2010 Apr 15): 4247-57.

    Lung mesenchymal expression of Sox9 plays a critical role in tracheal development.
    Warburton D
    BMC biology 11. (2013 Nov 25): 117.

    Multiple roles and interactions of Tbx4 and Tbx5 in development of the respiratory system.
    Papaioannou VE
    PLoS genetics 8.8 (2012): e1002866.

    Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis.
    Schiller HB
    Nature communications 11.1 (2020 Jul 16): 3559.

    An important role of the SDF-1/CXCR4 axis in chronic skin inflammation.
    Detmar M
    PloS one 9.4 (2014): e93665.

    Epithelial β1 integrin is required for lung branching morphogenesis and alveolarization.
    Zent R
    Development (Cambridge, England) 141.24 (2014 Dec): 4751-62.

    Two nested developmental waves demarcate a compartment boundary in the mouse lung.
    Chen J
    Nature communications 5. (2014 May 29): 3923.

    Dlg5 maintains apical aPKC and regulates progenitor differentiation during lung morphogenesis.
    Vasioukhin V
    Developmental biology 377.2 (2013 May 15): 375-84.

    Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2.
    Xia L
    Nature 502.7469 (2013 Oct 3): 105-9.

    Alveolar epithelial cell therapy with human cord blood-derived hematopoietic progenitor cells.
    Padbury JF
    The American journal of pathology 178.3 (2011 Mar): 1329-39.

    Sox2 activates cell proliferation and differentiation in the respiratory epithelium.
    Whitsett JA
    American journal of respiratory cell and molecular biology 45.1 (2011 Jul): 101-10.

    The chemokine CX3CL1 promotes trafficking of dendritic cells through inflamed lymphatics.
    Jackson DG
    Journal of cell science 126.Pt 22 (2013 Nov 15): 5259-70.

    Netrin-4 induces lymphangiogenesis in vivo.
    Li DY
    Blood 115.26 (2010 Jul 1): 5418-26.

    CD200-CD200R signaling suppresses anti-tumor responses independently of CD200 expression on the tumor.
    Meyaard L
    Oncogene 31.24 (2012 Jun 14): 2979-88.

    Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo.
    Schüler T
    European journal of immunology 40.9 (2010 Sep): 2391-400.

    An Adaptor Molecule Afadin Regulates Lymphangiogenesis by Modulating RhoA Activity in the Developing Mouse Embryo.
    Ogita H
    PloS one 8.6 (2013): e68134.

    Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans.
    Luther SA
    The American journal of pathology 178.4 (2011 Apr): 1662-75.

    Reproducible isolation of lymph node stromal cells reveals site-dependent differences in fibroblastic reticular cells.
    Turley SJ
    Frontiers in immunology 2. (2011): 35.

    Remodeling of the lymphatic vasculature during mouse mammary gland morphogenesis is mediated via epithelial-derived lymphangiogenic stimuli.
    Harvey NL
    The American journal of pathology 181.6 (2012 Dec): 2225-38.

    Hyaluronan and TLR4 promote surfactant-protein-C-positive alveolar progenitor cell renewal and prevent severe pulmonary fibrosis in mice.
    Noble PW
    Nature medicine 22.11 (2016 Nov): 1285-1293.

    Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program.
    Mäkinen T
    Nature communications 9.1 (2018 Apr 17): 1511.

    Genetic link between renal birth defects and congenital heart disease.
    Pazour GJ
    Nature communications 7. (2016 Mar 22): 11103.

    A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting.
    Zhou B
    Cell stem cell 28.6 (2021 Jun 3): 1160-1176.e7.

    Wnt3a mitigates acute lung injury by reducing P2X7 receptor-mediated alveolar epithelial type I cell death.
    Liu L
    Cell death & disease 5.6 (2014 Jun 12): e1286.

    Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5.
    Bonewald LF
    Methods in molecular biology (Clifton, N.J.) 816. (2012): 67-81.

    Serines in the intracellular tail of podoplanin (PDPN) regulate cell motility.
    Goldberg GS
    The Journal of biological chemistry 288.17 (2013 Apr 26): 12215-21.

    Tissue inflammation modulates gene expression of lymphatic endothelial cells and dendritic cell migration in a stimulus-dependent manner.
    Halin C
    Blood 118.1 (2011 Jul 7): 205-15.

    Lymph node-resident lymphatic endothelial cells mediate peripheral tolerance via Aire-independent direct antigen presentation.
    Engelhard VH
    The Journal of experimental medicine 207.4 (2010 Apr 12): 681-8.

    Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions.
    Turley SJ
    The Journal of experimental medicine 207.4 (2010 Apr 12): 689-97.

    Identification of salmonella pathogenicity island-2 type III secretion system effectors involved in intramacrophage replication of S. enterica serovar typhimurium: implications for rational vaccine design.
    Helaine S
    mBio 4.2 (2013 Apr 16): e00065.

    miR-31 functions as a negative regulator of lymphatic vascular lineage-specific differentiation in vitro and vascular development in vivo.
    Detmar M
    Molecular and cellular biology 30.14 (2010 Jul): 3620-34.

    Impaired thymic tolerance to α-myosin directs autoimmunity to the heart in mice and humans.
    Lipes MA
    The Journal of clinical investigation 121.4 (2011 Apr): 1561-73.

    Interleukin-7 is produced by afferent lymphatic vessels and supports lymphatic drainage.
    Halin C
    Blood 122.13 (2013 Sep 26): 2271-81.

    Novel role for ALCAM in lymphatic network formation and function.
    Halin C
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27.3 (2013 Mar): 978-90.

    Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues.
    Aruffo A
    The Journal of experimental medicine 176.5 (1992 Nov 1): 1477-82.

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