3H11
Catalog Fields
Product Name/ID: 3H11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: LAMA1/LAMB1/LAMC1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528342
Uniprot ID: F1NM47 Q01635 F1NI05
Entrez Gene ID: 374016 396478 424442
Clonality: Monoclonal
Immunogen: Retinal basal laminae (E10)
Clone:
Immunogen Sequence: full length protein
Myeloma Strain: X63Ag8.653
Epitope Mapped: No
Antigen Name: laminin-1, avian
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/8/2001
Antigen Molecular Weight: Apparent: 200 kDa (beta1 and gamma1) and 400 kDa (alpha1)
Depositor: Halfter, W.M.
Antigen Sequence:
Depositor Institution: University of Pittsburgh
Antigen Species: chicken
Depositor Notes: This antibody is avian specific. Use non-reducing conditions for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian
Additional Information: This antibody recognizes native embryonic chicken laminin.
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H11 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 3H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane.
Von Boxberg Y
The European journal of neuroscience 4.9 (1992): 840-852.