FE-A5
Catalog Fields
Product Name/ID: FE-A5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_531818
Clonality: Monoclonal
Immunogen: Rat testicular cell lysate
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: Embryonal carcinoma cell surface carbohydrate
Epitope Location or Sequence: Carbohydrate moiety Gal b 1-4 G1cNAc b 1-3 R
Alternate Antigen Name:
Deposit Date: 9/12/1990
Antigen Molecular Weight:
Depositor: Fenderson, B. / Eddy, M.
Antigen Sequence:
Depositor Institution: Thomas Jefferson University
Antigen Species: Rat
Depositor Notes: This antibody recognizes a carbohydrate epitope found on glycolipids and glycoproteins: Gal b 1-4 G1cNAc b 1-3 R. This carbohydrate moiety is found on the surface of mouse NULLI-SCC1 and F9 testis embryonal carcinoma cells. Bind of FE-A5 is strongly inhibited by the presence of N-acetyllactoseamine, lactose and D-Galactose.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Fish, Mammal, Mouse, Ovine, Rat
Additional Information: FE-A5 has been successfully used in TLC overlay applications (PMID: 26405105).
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
FE-A5 was deposited to the DSHB by Fenderson, B. / Eddy, M. (DSHB Hybridoma Product FE-A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
8 References
Initial Publication
Immunohistochemical localization of two monoclonal antibody-defined carbohydrate antigens during early murine embryogenesis.
Eddy EM
Developmental biology 100.2 (1983 Dec): 318-27.