F55A12
Catalog Fields
Product Name/ID: F55A12
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Nkx6-1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Entrez Gene ID: 65193
Clonality: Monoclonal
Immunogen: GST- Nkx6.1 C-terminus: a.a. 299-365) developmental stage E9
Clone:
Immunogen Sequence: a.a. 299-365
Myeloma Strain: Fox-NY
Epitope Mapped: Yes
Antigen Name: Homeobox protein Nkx-6.1; Nkx6.1
Epitope Location or Sequence: C-terminal a.a. 299-365, DDDYNKP
Alternate Antigen Name:
Deposit Date: 3/15/2006
Antigen Molecular Weight: 44-46 kDa
Depositor: Madsen, O.D.
Antigen Sequence:
Depositor Institution: Hagedorn Research Institute
Antigen Species: Rat
Depositor Notes: This antibody is also available from the Beta Cell Consortium at http://www.betacell.org/ab/ab_view.php?AB_ID=2023
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Bovine, Human, Mouse, Porcine, Rat
Additional Information:
Recommended Applications: FACS, FFPE, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F55A12 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F55A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
Jorgensen MC
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.