DSAP47-1 (nc46)
Catalog Fields
Product Name/ID: DSAP47-1 (nc46)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Sap47
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2617213
Uniprot ID: A0A0B4LH71
Entrez Gene ID: 41938
Clonality: Monoclonal
Immunogen: Brain homogenate
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X.63-Ag8.653
Epitope Mapped: Yes
Antigen Name: Synapse-associated protein 47kD
Epitope Location or Sequence: N-terminal decapeptide FSGLTNQFTS
Alternate Antigen Name:
Deposit Date: 2/5/2011
Antigen Molecular Weight: 47 kDa
Depositor: Buchner, E. / Hofbauer, A.
Antigen Sequence:
Depositor Institution: University of Wuerzburg - Germany
Antigen Species: Drosophila
Depositor Notes: The antibody nc46 recognizes 9 SAP47 isoforms (see PMID: 15117418).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Ant, Drosophila, Fish, Insects, Mosquito
Additional Information: RRID:AB_2617213. FBgn0013334.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSAP47-1 (nc46) was deposited to the DSHB by Buchner, E. / Hofbauer, A. (DSHB Hybridoma Product DSAP47-1 (nc46))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
22 References
Initial Publication
The sap47 gene of Drosophila melanogaster codes for a novel conserved neuronal protein associated with synaptic terminals.
Buchner E
Brain research. Molecular brain research 32.1 (1995 Aug): 45-54.