BC-12.CA4
Catalog Fields
Product Name/ID: BC-12.CA4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_10570196
Clonality: Monoclonal
Immunogen: PBS cell surface washings of plate culture of Botrytis cinerea + hybridoma supernatant from nonspecific cell line BC-EC7 in 1:1 ratio (Meyer & Dewey, 2000)
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: Botrytis cinerea
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/9/2010
Antigen Molecular Weight: 100-200 kDa
Depositor: Dewey, F.M.
Antigen Sequence:
Depositor Institution: University of Oxford
Antigen Species: Botrytis cinerea
Depositor Notes: Water soluble, thermostable glycoprotein that is not metabolized during fermentation of infected grape juice. Near-genus specific, recognizes all species of Botrytis and species of related genus Sclerotinia(fungi) plus Magnapothe grisea, but does not recognize other fungi including yeasts or extracts from uninfected plants. Cell binding: binds to extracellular matrix of hyphal walls species of Botrytis and Sclerotinia (fungi), does not bind to asexual spores (conidia) or sexual spores (ascospores). Immunohistology: has been used in immunofluorescence and TEM studies to visualize the fungal hyphae in vitro and in vivo. IHC note: binding site is carbohydrate containing rhamnose
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Botrytis cinerea
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BC-12.CA4 was deposited to the DSHB by Dewey, F.M. (DSHB Hybridoma Product BC-12.CA4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
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