7D6
Catalog Fields
Product Name/ID: 7D6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: CDH1
Ab Isotype: MIgG1
Gene Symbol:
Entrez Gene ID: 415860
Clonality: Monoclonal
Immunogen: 81 kDa N-terminal tryptic fragment of L-CAM from chicken liver plasma membranes.
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3U
Epitope Mapped: Yes
Antigen Name: L-CAM/E-cadherin, chicken
Epitope Location or Sequence: Extracellular domain
Alternate Antigen Name:
Deposit Date: 5/13/1991
Antigen Molecular Weight: Predicted: 98 kDa; Apparent: 124 kDa
Depositor: Gallin, W.
Antigen Sequence:
Depositor Institution: University of Alberta
Antigen Species: Chicken
Depositor Notes: Does not cross-react with any mammalian species tested, nor with Xenopus laevis, nor with any invertebrate species tested. Doesn't appear to work for western blotting. Doesn't appear to affect L-CAM function. Stains nearly all epithelial cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken
Additional Information:
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7D6 was deposited to the DSHB by Gallin, W. (DSHB Hybridoma Product 7D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
17 References
Initial Publication
Characterization of L-CAM, a major cell adhesion molecule from embryonic liver cells.
Cunningham BA
Proceedings of the National Academy of Sciences of the United States of America 80.4 (1983 Feb): 1038-42.