12/21/1-C-6
Catalog Fields
Product Name/ID: 12/21/1-C-6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ACAN
Ab Isotype: MIgG1
Gene Symbol:
Entrez Gene ID: 176
Clonality: Monoclonal
Immunogen: Reduced and alkylated G3 domain at the C-terminus
Clone:
Immunogen Sequence: Protein domain
Myeloma Strain: X63-Ag8.653
Epitope Mapped: No
Antigen Name: Aggrecan core protein
Epitope Location or Sequence:
Alternate Antigen Name: aggrecan
Deposit Date: 6/1/1986
Antigen Molecular Weight: 221.9kDa
Depositor: Caterson, B.
Antigen Sequence:
Depositor Institution: Cardiff University, Nat. Institute of Dental Research, NIH
Antigen Species: Rat
Depositor Notes: To expose the core protein epitope, the tissue must be reduced and alkylated. Treat the tissue with 10mM dithiothreitol in TBS pH 7.4 for 2 hr at 37ºC for 2 hr. Follow by alkalation with 40 mM iodoacetamide in PBS for 2 hr.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Bovine, Canine, Chicken, Fish, Human, Pig, Rat
Additional Information: This antibody has been used successfully on native dot blots (PMID:7632088). RRID: AB_531912
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12/21/1-C-6 was deposited to the DSHB by Caterson, B. (DSHB Hybridoma Product 12/21/1-C-6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
26 References
Initial Publication
Monoclonal antibodies directed against epitopes within the core protein structure of the large aggregating proteoglycan (aggrecan) from the swarm rat chondrosarcoma.
Caterson B
Archives of biochemistry and biophysics 298.2 (1992 Nov 1): 349-60.